Computational Identification and Characterization of a Promiscuous T-Cell Epitope on the Extracellular Protein 85B of Mycobacterium spp. for Peptide-Based Subunit Vaccine Design

Figure 2

Multiple alignment of protein sequences of Ag85B from Mycobacterium spp. Accession numbers of Ag85B from M. tuberculosis were NP_216402 (M. tuberculosis_1 H37Rv), NP_336393 (M. tuberculosis_2 CDC1551), 1F0PA (M. tuberculosis_3), ZP_07422952 (M. tuberculosis_4 SUMu003), ZP_02552646 (M. tuberculosis_5 H37Ra), 1F0N_A (M. tuberculosis_6), and AAO62005 (M. tuberculosis_7). Accession numbers of Ag85B from M. bovis were YP_978013 (M. bovis_1 BCG str.) and AAA25359 (M. bovis_2). Accession numbers of Ag85B from M. intracellulare were YP_005338175 (M. intracellulare _1 ATCC), BAA03981 (M. intracellulare_2 ATCC), and BAA03243 (M. intracellulare_3). Accession numbers of Ag85B from M. avium were Q06947 (M. avium_1) and AAM21939 (M. avium_2), and those of Ag85B from M. kansasii were ZP_04749192 (M. kansasii ATCC 12478). The best epitope based on conservation and NetMHCII 2.2 analysis is shown in the box. The epitope sequence from M. tuberculosis_1 H37Rv is underlined. Nonidentical epitope sequences are shaded and the amino acid residues for which the epitope varies are bold, italic, and underlined.