Rapid Identification and Isolation of Inhibitors of Rat Lens Aldose Reductase and Antioxidant in Maackia amurensis

Table 3

Antioxidant and aldose reductase inhibitory effects in rat lens of nine compounds isolated from the ethyl acetate fraction of Maackia amurensis.

Isolated compounds	Antioxidant		RLAR inhibition
	Quantitative reduction (%)	IC50 (μM)	Inhibition (%)	IC50 (μM)
Chlorogenic acid (1)	17.09	27.23 ± 0.17	51.02	4.2 ± 3.20
Formononetin-7-O-β-D-glucosyl glucoside (2)	1.04	>500	2.32	>500
trans-Ferulic acid (3)	20.06	13.51 ± 0.04	5.21	>500
Tectoridin (4)	8.43	>500	43.37	50.4 ± 2.17
Piceatannol (5)	34.68	6.73 ± 0.04	5.47	>500
Resveratrol (6)	29.96	11.05 ± 0.04	29.31	117.6 ± 3.12
Daidzein (7)	0.48	>500	28.14	151.9 ± 2.91
Genistein (8)	2.24	>500	38.54	57.1 ± 3.23
Formononetin (9)	0.69	>500	30.11	69.2 ± 2.57
Trolox	—	16.83 ± 0.06	—	—
Quercetin	—	—	—	10.1 ± 2.18

Quantitative reduction was defined as the amount of decrease in ABTS-offline HPLC.
The IC₅₀ value was defined as the concentration of the 50% inhibition.
Inhibition was defined as the amount of efficacy in HPLC microfractionation.
Trolox was used as positive control for ABTS assay.
Quercetin was used as positive control for RLAR inhibitory activity measurement.