A Robust PCR Protocol for HIV Drug Resistance Testing on Low-Level Viremia Samples

Table 1

Primers used for RT-PCR and nested PCR.

	Primer name	HXB2 loci	Sequences 5′ – 3′
RT-PCR	PR_2071_F1	2071–2095	GAR AGA CAG GCT AAT TTT TTA GGG A
	RT_3370_R1	3348–3370	ATC CCT GCA TAA ATC TGA CTT GC
Nested PCR [one-amplicon]	PR_2243_F2	2243–2266	CTT TAR CTT CCC TCA RAT CAC TCT
	RT_3326_R2	3304–3326	CTT CTG TAT GTC ATT GAC AGT CC
Nested PCR [two-amplicon]	PR_F1	2243–2266	CTT TAR CTT CCC TCA RAT CAC TCT
	PR_R1	2761–2785	TCT CTG AAA TCT ACT AAT TTY CTC C
	RT_F2	2692–2713	CAA AAA TTG GGC CTG AAA ATC C
	RT_R2	3249–3270	CTG TCC ATT TRT CAG GAT GRA

Notes. The PR_2071_F1 primer was from Dr. Richard Harrigan’s Lab (University of British Columbia, Canada).