The Combination of Computational and Biosensing Technologies for Selecting Aptamer against Prostate Specific Antigen

Table 3

Second-generation sequences and analysis and calculation results.

Name	Sequence (5′-3′)	Minimum free energy (kcal/mol)	Dot-bracket format	ZDOCK score	ZRANK score	Rank
PSAG21	TTTTTCTGGAATGATTAACGTCGTGGCCCTTT		.......((.(((((....)))))..))....	43.44
PSAG22	TTTTTACTTAATGCGGTCCCGGGGGGCTCTTT		..............(((((....)))))....	45.77
PSAG23	TTTTTCTGGAATGATTACGTTTTTTGGCCTTT		.......((((((....)))))).........	41.61
PSAG24	TTTTTAATATCCGGGTTCCCGGTTGTCTCTTT		......(((.((((....)))).)))......	17.54		3
PSAG25	TTTTTCTGGAATGATTTCCCGGTTTGGCCTTT		.......((((....)))).(((...)))...	45.69
PSAG26	TTTTTACGCACCGGGTACGTTTTTGTCTCTTT		............(((.(((....))))))...	44.63
PSAG27	TTTTTACTTAATGCGTACGTTTTTTGGCCTTT		................................	N.A.	N.A.
PSAG28	TTTTTAATATCCGGGGAACGTCGTGGCCCTTT		............(((..((...))..)))…	42.64		1
PSAG29	TTTTTACTTAATGCGGACGTTTTTTGGCCTTT		............((.((......)).))....	44.51
PSAG210	TTTTTACGCACCGGGTAACGTCGTGGCCCTTT		............((((.((...)).))))...	42.61
PSAG211	TTTTTACTTAATGAATACGTTTTTTGGCCTTT		................................	N.A.	N.A.
PSAG212	TTTTTACGCACCGGGTACGTTTTTTGGCCTTT		............((((.((.....))))))..	40.41		2