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BioMed Research International
Volume 2017 (2017), Article ID 5092704, 10 pages
https://doi.org/10.1155/2017/5092704
Research Article

Technological and Theoretical Aspects for Testing Electroporation on Liposomes

1Center for Life Nano Science@Sapienza, Istituto Italiano di Tecnologia, 00161 Rome, Italy
2Department of Information Engineering, Electronics and Telecommunication (DIET), University of Rome “La Sapienza”, 00184 Rome, Italy
3Vectorology and Antitumor Therapies, UMR 8203, CNRS, Univ. Paris-Sud, Gustave Roussy, Université Paris-Saclay, 94805 Villejuif, France

Correspondence should be addressed to Micaela Liberti; ti.1amorinu@itrebil.aleacim

Received 6 November 2016; Accepted 15 February 2017; Published 14 March 2017

Academic Editor: Hannes Stockinger

Copyright © 2017 Agnese Denzi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Recently, the use of nanometer liposomes as nanocarriers in drug delivery systems mediated by nanoelectroporation has been proposed. This technique takes advantage of the possibility of simultaneously electroporating liposomes and cell membrane with 10-nanosecond pulsed electric fields (nsPEF) facilitating the release of the drug from the liposomes and at the same time its uptake by the cells. In this paper the design and characterization of a 10 nsPEF exposure system is presented, for liposomes electroporation purposes. The design and the characterization of the applicator have been carried out choosing an electroporation cuvette with 1 mm gap between the electrodes. The structure efficiency has been evaluated at different experimental conditions by changing the solution conductivity from 0.25 to 1.6 S/m. With the aim to analyze the influence of device performances on the liposomes electroporation, microdosimetric simulations have been performed considering liposomes of 200 and 400 nm of dimension with different inner and outer conductivity (from 0.05 to 1.6 S/m) in order to identify the voltage needed for their poration.