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BioMed Research International
Volume 2017 (2017), Article ID 5103803, 9 pages
Research Article

Analysis of Differentially Expressed Proteins in Mycobacterium avium-Infected Macrophages Comparing with Mycobacterium tuberculosis-Infected Macrophages

Department of Biochemistry and Molecular Biology, Guangxi Medical University, Key Laboratory of Biological Molecular Medicine Research of Guangxi University, Nanning, Guangxi 530021, China

Correspondence should be addressed to Min Ling

Received 11 December 2016; Revised 21 February 2017; Accepted 6 March 2017; Published 10 May 2017

Academic Editor: Heide Schatten

Copyright © 2017 Dongjun Yang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Mycobacterium avium (MA) belongs to the intracellular parasitic bacteria. To better understand how MA survives within macrophages and the different pathogenic mechanisms of MA and Mycobacterium tuberculosis (MTB), tandem mass tag (TMT) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis have been used to determine the proteins which are differentially expressed in MA-infected and MTB-infected macrophages. 369 proteins were found to be differentially expressed in MA-infected cells but not in MTB-infected cells. By using certain bioinformatics methods, we found the 369 proteins were involved in molecular function, biological process, and cellular component including binding, catalytic activity, metabolic process, cellular process, and cell part. In addition, some identified proteins were involved in multiple signaling pathways. These results suggest that MA probably survive within macrophages by affecting the expression of some crucial proteins.