Developmental Testicular Expression, Cloning, and Characterization of Rat HDAC6 In Silico

Figure 1

Western blot analysis for HDAC6. Western blot analysis was done to detect HDAC6 in multiple rat tissues and in testis from day 0 until day 90. The transcript for HDAC6 in the testis on these days was detected by RT-PCR. A band of 130 kDa was observed in testis, epididymis (cauda and caput region), prostrate, seminal vesicles, brain, adrenal, lungs, and liver (a). A 209 bp band indicative of the hdac6 transcript is seen in the testis from birth till day 90 (b). The upper panel, middle panel, and lower panel show the 130 kDa band of HDAC6, 55 kDa band for acetylated alpha-tubulin (Ac α-tubulin), and alpha-tubulin (α-tubulin), respectively, from birth to 90 d (c). “Reagent” control (RC) as well as “No RT” (NRT) control were incorporated to account for nonspecific amplification, if any.

(a)

(b)

(c)