Developmental Testicular Expression, Cloning, and Characterization of Rat HDAC6 In Silico

Figure 2

Amplification of hdac6 cDNA from rat testis. RNA was isolated from testis of 28-day-old adult Holtzman rat, reverse transcribed, and the cDNA amplified by PCR using primers designed for accession number XM_228753.8. (a). “Reagent” control (RC) as well as “no reverse transcriptase” (No RT) control were incorporated to account for nonspecific amplification. Lane 1: 1 Kb plus ladder; Lanes 2, 3, and 5: amplified Hdac6; Lane 4: No RT control; Lane 6: reagent control. Rat Hdac6 cDNA is ~3.5 kb. Gel extracted PCR product (b). The sequences obtained were aligned using BLAST tool. Four nucleotide differences were observed in different regions of the nucleotide sequences with respect to XM_228753.8.

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