Developmental Testicular Expression, Cloning, and Characterization of Rat HDAC6 In Silico

Figure 3

Amplification, sequencing and analysis of exons 2, 5, 7, and 26. Exons 2, 5, 7, and 26 were amplified by PCR using primers specific to these regions (a). L-100 bp ladder. PCR products were sequenced by Sangers method. All the 4 nucleotide differences were also present at genomic DNA level. Representative image of exon specific amplification (b). Alignment of exon specific sequencing results with human, mouse, and rat (predicted) sequence shows 2 amino acid residue changes (c).

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(b)

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