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BioMed Research International
Volume 2017, Article ID 5185034, 7 pages
https://doi.org/10.1155/2017/5185034
Research Article

Elevated Foxp3/CD8 Ratio in Lung Adenocarcinoma Metastatic Lymph Nodes Resected by Transcervical Extended Mediastinal Lymphadenectomy

1Department of Internal Medicine, Pulmonology and Allergology, Medical University of Warsaw, Warsaw, Poland
2Laboratory of Flow Cytometry and Hematology, Department of Internal Medicine and Hematology, Military Medical Institute, Warsaw, Poland
3Department of Pathology, Pulmonary Hospital, Zakopane, Poland
4Department of Pathology, Medical University of Warsaw, Warsaw, Poland
5Department of Thoracic Surgery, Pulmonary Hospital, Zakopane, Poland

Correspondence should be addressed to Joanna Domagala-Kulawik; moc.liamg@kiwalukalagamod

Received 7 March 2017; Revised 20 June 2017; Accepted 5 July 2017; Published 2 August 2017

Academic Editor: Torsten Goldmann

Copyright © 2017 Joanna Domagala-Kulawik et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A balance between tumor invasion and immune defence system is widely investigated. Objective. The aim of this study was to evaluate lymphocyte phenotype in lymph nodes (LNs) of patients with lung cancer in relation to the presence of metastases. Methods. We investigated 364 LNs resected by transcervical extended mediastinal lymphadenectomy (TEMLA) of 49 patients with squamous cell carcinoma (SCC) or adenocarcinoma (AD) with (A) and without metastases (B). Expression of CD4, CD8, CD25, CTLA-4, and Foxp3 was assessed by immunohistochemical staining. Results. We observed a strong nuclear staining for Foxp3 in lymphocytes and cancer cells and strong membranous/cytoplasmatic reaction for CD4 and CD8, but low for CD25 and CTLA-4. There were significantly higher proportions of CD8+ cells in AD (B) versus AD (A) LNs (80% versus 52.5%, ). The Foxp3/CD8 ratio was higher in AD (A) versus AD (B) LNs (0.4 versus 0.25, ). No significant differences in the cell markers expression in SCC LNs were found. Conclusion. Significant differences in lymphocyte phenotype in AD may indicate an exceptional biology of this type of lung cancer. TEMLA resected LNs may serve as valuable samples for evaluation of immune status in lung cancer patients.