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BioMed Research International
Volume 2017, Article ID 5191046, 8 pages
Research Article

Gibberellic Acid Production by Different Fermentation Systems Using Citric Pulp as Substrate/Support

Bioprocess Engineering and Biotechnology Department, Federal University of Paraná (UFPR), Curitiba, PR, Brazil

Correspondence should be addressed to Cristine Rodrigues; moc.liamg@robalenitsirc

Received 30 March 2017; Revised 30 June 2017; Accepted 17 July 2017; Published 7 September 2017

Academic Editor: Hwa-Liang Leo

Copyright © 2017 Juliana de Oliveira et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


Gibberellic acid (GA3) is an important phytohormone, a member of gibberellins family, which acts as a promoter and regulator of plant growth. This study aimed to evaluate GA3 production by Fusarium moniliforme LPB03 and Gibberella fujikuroi LPB06 using different techniques of fermentation, solid state fermentation (SSF), submerged fermentation (SmF), and semisolid state fermentation (SSSF), and different types of bioreactors. In all techniques, citric pulp (CP), a subproduct obtained from the extraction of orange juice, was employed as the substrate/support. GA3 production by SSF reached 7.60 g kg−1 and 7.34 g kg−1 in Erlenmeyer flasks and column bioreactors, respectively. For SmF, the highest concentration of GA3 obtained was 236.00 mg L−1 in Erlenmeyer flasks, 273.00 mg L−1 in a 10 L stirred tank reactor (STR), and 203.00 mg L−1 in a 1.5 L bubble column reactor (BCR). SSSF was conducted with a CP suspension. In this case, GA3 concentration reached 331.00 mg L−1 in Erlenmeyer flasks and 208 mg L−1 in a BCR. The choice of the fermentation technique is undoubtedly linked to the characteristics and productivity of each process. The methods studied are inexpensive and were found to produce good proportions of GA3, making them suitable for several applications.