Expression of MicroRNA-29a Regulated by Yes-Associated Protein Modulates the Neurite Outgrowth in N2a Cells

Figure 1

YAP increases the expression of miR-29a and decreases PTEN in the N2a cells. (a) Real-time PCR analysis shows relative expression of miR-29 family with YAP overexpression and knockdown in N2a cells. The control group is transfected with a blank vector and the KD group is treated with sh-YAP. Mean ± SD (). (b) There are changes in levels of PTEN protein expression when YAP OE and YAP KD, and the GAPDH served as an internal control. The blank vector is used as a control. (c) There is the quantification of densitometric levels of PTEN. Mean ± SD (). An ANOVA test is used here. , . (d) Real-time PCR test for the relative expression of miR-29a mimic and miR-29a inhibitor. We use miR-29a inhibitor control as the baseline. Mean ± SD (). An ANOVA test is used here. , .

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