Research Article

Expression of MicroRNA-29a Regulated by Yes-Associated Protein Modulates the Neurite Outgrowth in N2a Cells

Figure 4

The effect of YAP and Akt pathway on axonal growth. (a) There is the expression of Akt and Akt phosphorylation in YAP OE cell treated with or without PI3-kinase inhibitor (LY294002). (b) There is the quantification of densitometric levels. Mean ± SD (). The figure shows the similar expression of Akt protein in LY294002 (+) group and LY294002 (−) group, but p-Akt almost disappeared in LY294002 (+) group. Student’s -test is used here. . (c) Immunofluorescence is used to observe the neurite outgrowth at different conditions. Original cells are showed with no axon outgrowth on the first day (A). After 72 hours of treatment with YAP only, we see some growth in axon outgrowth (B). But treated with YAP, while adding LY294002 at the same time, we find a significant inhibitory effect in axon outgrowth compared with the YAP group (C).
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