Postneonatal Mortality and Liver Changes in Cloned Pigs Associated with Human Tumor Necrosis Factor Receptor I-Fc and Human Heme Oxygenase-1 Overexpression

Figure 3

Transgenic pig harboring 15 transgenes shows accumulation of hemorrhagic apoptosis in liver. After death of transgenic pigs, autopsy was performed. (a)-(b) Representative gross images of liver of C15-shTNFRI-Fc/hHO-1 pigs. (a′)-(b′) Representative images of hematoxylin and eosin (H&E) stating of liver. Original magnification, 200x. (a′′)-(b′′) Original magnification, 400x. (c)–(f) The expression of several genes in liver of a C4-shTNFRI-Fc/hHO-1 transgenic pig and seven C15-shTNFRI-Fc/hHO-1 pigs. (c) Ferritin heavy chain, (d) ferritin light chain, (e) transferrin, and (f) inducible NOS. Data were shown as means ± standard deviations of six replicates. Data followed by different letters are significantly different (). (g)-(h) The quantitative analysis of H₂O₂ (g) and superoxide dismutase (h) in produced piglets liver. Data were shown as means ± standard deviations of six replicates. Data followed by different letters are significantly different ().