Research Article

Overhydroxylation of Lysine of Collagen Increases Uterine Fibroids Proliferation: Roles of Lysyl Hydroxylases, Lysyl Oxidases, and Matrix Metalloproteinases

Figure 5

(a) Expression of three different isoforms of LH1, LH2, and LH3. The mRNA levels in samples of UF (F) and adjacent myometrial tissues (M) () were measured by quantitative real-time RT-PCR and were normalized to GAPDH mRNA levels. All values in fibroid tissues were described as fold differences compared to those measured in the corresponding myometrial tissues. Results are presented as mean ± SD. indicates a significant difference from control myometrium (). (b) PCR amplification of different isoforms of LH2: cDNA from samples of UF and normal myometrial tissues were PCR amplified using primers for LH2a and LH2b. Aliquots of each amplified sample were subjected to electrophoresis in a 2% agarose gel containing ethidium bromide. The expected band sizes were 145 bp for LH2a and 208 bp for LH2b.
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