Coutilization of D-Glucose, D-Xylose, and L-Arabinose in Saccharomyces cerevisiae by Coexpressing the Metabolic Pathways and Evolutionary Engineering

Figure 4

The metabolic capacities of BSW4XA3 and BSW3AP on mixed sugars of D-glucose, D-xylose, and L-arabinose. (a) The growth curves of strains BSW3AP (△) and BSW4XA3 (⋄). (b) The sugar utilization and sugar alcohols production of BSW3AP. (c) The sugar utilization and sugar alcohols production of BSW4XA3. D-Glucose (◆), D-xylose (■), L-arabinose (▲), ethanol (●), L-arabitol (×), and xylitol (+). The strains were preincubated in SC-Leu-Ura medium containing 20 g L⁻¹ D-glucose for 24 h and then transferred using 20 g L⁻¹ D-glucose, 20 g L⁻¹ D-xylose, and 20 g L⁻¹ L-arabinose as the carbon sources to incubate for 24 h. The cells were then collected and used for fermentation in 40 mL SC-Leu-Ura medium with 20 g L⁻¹ D-glucose, 20 g L⁻¹ D-xylose, and 20 g L⁻¹ L-arabinose at 30°C, 200 r min⁻¹, and the initial OD₆₀₀ was 1.5. The data are the averages of three independent tests.

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