Identification of Significant Pathways Induced by PAX5 Haploinsufficiency Based on Protein-Protein Interaction Networks and Cluster Analysis in Raji Cell Line

Figure 1

Mutation type of pax5 and identification. (a) The construction of pax5 and mutation type of pax5. E5 was the target of gene editing operation, and sequencing analyses identified knocked out regions of E5. (b) Protein level of pax5 tested by western blot. Protein level of pax5 in MUT1 and MUT2 was less than 50% of that in WT. E: exon of pax5; PD: paired box domain; OP: octapeptide domain; HD: homeodomain; TA: transactivation domain; ID: inhibitory domain; WT: wild-type clone; MUT: mutation clone. means   .

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