Efficient Expression of Maltohexaose-Forming α-Amylase from Bacillus stearothermophilus in Brevibacillus choshinensis SP3 and Its Use in Maltose Production

Table 2

Experimental plan and corresponding α-amylase activity.

Run order	Glucose (g·L^−1)		Yeast extract powder (g·L^−1)		Proline (g·L^−1)		α-Amylase activity (×10^3 U·mL^−1)
	Code A	A	Code B	B	Code C	C	Predicted	Actual
1	1	24.93	−1	10.07	1	12.16	10.03
2	−1	10.07	1	24.93	−1	3.84	6.50
3	0	17.50	0	17.50	0	8.00	16.89
4	0	17.50	0	17.50	−1.68	1.00	7.67
5	0	17.50	0	17.50	0	8.00	16.89
6	1	24.93	−1	10.07	−1	3.84	8.85
7	0	17.50	0	17.50	0	8.00	16.89
8	1	24.93	1	24.93	−1	3.84	8.85
9	1	24.93	1	24.93	1	12.16	6.66
10	−1	10.07	−1	10.07	1	12.16	10.20
11	0	17.50	0	17.50	0	8.00	16.89
12	0	17.50	−1.68	5.00	0	8.00	9.82
13	0	17.50	0	17.50	0	8.00	16.89
14	0	17.50	0	17.50	1.68	15.00	9.54
15	0	17.50	1.68	30.00	0	8.00	7.69
16	−1	10.07	1	24.93	1	12.16	6.48
17	−1	10.07	−1	10.07	−1	3.84	5.35
18	1.68	30.00	0	17.50	0	8.00	8.69
19	−1.68	5.00	0	17.50	0	8.00	6.59