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BioMed Research International
Volume 2017 (2017), Article ID 5642535, 6 pages
https://doi.org/10.1155/2017/5642535
Research Article

Immunocytochemistry Improving the Diagnosis of Trichomonas vaginalis Infections

1Department of Parasitology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
2Department of Pathology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
3Department of Gynecology and Obstetrics, Universidade Federal de Minas Gerais, Belo Horizonte, MG, Brazil
4IPSEMG Hospital, Belo Horizonte, MG, Brazil
5Universidade de Itaúna, Itaúna, MG, Brazil

Correspondence should be addressed to Maria Aparecida Gomes; rb.gmfu.bci@semogam

Received 29 December 2016; Revised 14 February 2017; Accepted 26 February 2017; Published 23 March 2017

Academic Editor: Marlene Benchimol

Copyright © 2017 Thaisa Helena S. Fonseca et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of this study was to evaluate the immunocytochemistry (ICC) to diagnose trichomoniasis, particularly asymptomatic infections. By culture serial dilutions, ICC was able to detect 1 trophozoite/mL, while the culture was positive up to 100 trophozoites/mL. The ICC in vivo detection capability was assessed in vaginal secretions of mice experimentally infected and in vaginal swabs from asymptomatic HIV-positive pregnant women compared with culture. All vaginal secretion samples from mice were positive according to both methods. Swabs from fifty-five asymptomatic women were positive in four (7.27%) of them by culture. Beyond these four, another ten (25.45%) women were positive by immunocytochemistry, proving their higher sensitivity (), noticing 3.5 times more positives. ICC had better performance in both successive dilutions as in asymptomatic women, showing higher sensitivity and specificity. In this way, its facility of execution and cost-effectiveness support its practicality, as a routine procedure to diagnose trichomoniasis not only when the parasite load is lower but probably in all clinical scenarios.