Pharmacokinetic and Toxicological Evaluation of a Zinc Gluconate-Based Chemical Sterilant Using In Vitro and In Silico Approaches

Figure 3

Infertile genotoxicity and cytotoxicity evaluation using RAW267.4 macrophage cell lineage. There are three curves showing the percentage of micronucleated cells (MN) in apoptosis (Apo) and necrosis (Nec). It can be observed that, at 12 mM, there is predominance of micronucleated cells (85/1000 cells), at 30 mM, cells in apoptosis predominate (143/1000 cells) and, at 60 mM of Infertile, there were 318 cells in necrosis/1000. It is important to note that when an event prevails, others tend to diminish. It is possible to observe that there is a turnover among the cell death events ( < 0.01 versus negative control; in triplicate; one-way ANOVA followed by Tukey’s post hoc test).