Rapid Elimination of Blood Alcohol Using Erythrocytes: Mathematical Modeling and In Vitro Study

Figure 8

Changes in the ethanol concentration during incubation of alcocytes at different pyruvate concentrations. (■) Pyruvate concentration was maintained at 100 µM; (○) initial pyruvate concentration was 50 mM (as well as 10 mM, or 1 mM, not shown). At 100 µM and 50 mM pyruvate, the initial rate of ethanol oxidation was about 30 mmol/·h. Typical experiments are presented. In the mathematical model (lines), ADH activity of 83 nkatals/ml, ALD activity of 33 nkatals/ml, LDH activity of 225 nkatals/ml, and hematocrit of 21% were used.