Renoprotective Effects of Aldose Reductase Inhibitor Epalrestat against High Glucose-Induced Cellular Injury

Figure 1

Effect of high glucose (30 mM) on NRK-52E cells viability and relative mitochondrial membrane potential. (a) Cell viability of low passage number cells (P2–P6) after 24 h, 48 h, and 72 h exposure to high glucose as determined by MTT assay. Absorbance values were normalized to control group and expressed as percentage Mean ± SEM (n = 4–7). compared to control 48 h. compared to control 72 h. (b) Effect of high glucose concentration on relative mitochondrial membrane potential in NRK-52E cells after 24 and 48 h exposure using JC-1 staining. Ratios obtained from fluorescence values of J-aggregates (excitation/emission 488/529 nm) and that of monomers (excitation/emission 488/590 nm) were expressed as percentage Mean ± SEM compared to control. compared to control 24 h.

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