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BioMed Research International
Volume 2017 (2017), Article ID 6160785, 13 pages
https://doi.org/10.1155/2017/6160785
Research Article

Polysaccharides from Trichosanthes Fructus via Ultrasound-Assisted Enzymatic Extraction Using Response Surface Methodology

1College of Biotechnology and Food Engineering, Huanghuai University, Zhumadian 463000, China
2College of Chemistry and Pharmaceutical Engineering, Huanghuai University, Zhumadian 463000, China
3College of Liberal Arts, Department of Biology, Mercer University, Macon, GA, USA

Correspondence should be addressed to Jiayang Liu; ude.agu@uilyj

Received 1 May 2017; Accepted 20 August 2017; Published 25 September 2017

Academic Editor: José D. Fontana

Copyright © 2017 Fujia Chen et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

An efficient procedure for ultrasound-assisted enzymatic extraction of crude polysaccharides from Trichosanthes Fructus (crude TFP) using response surface methodology (RSM) was developed. The Box–Behnken design was applied to optimize the effects of pH (), enzyme amount (), extraction temperature (), and liquid-to-solid ratio () on the extraction. The statistical analysis indicated that the independent variables (, , and ), the quadratic coefficients (, , , and ), and the interaction coefficient () had significant impact on the yield of crude TFP. The optimal conditions were determined as follows: pH 4.5, enzyme amount 5000 u/g, extraction temperature 45°C, and liquid-to-solid ratio 30 ml/g. The experimental yield of crude TFP was 6.58%, which was very close to the predicted yield of 6.71%. TFPI was then purified and characterized with Sephadex G-100 column, UV-Vis, GPC, and FT-IR. The average molecular weight of TFPI was calculated to be 1.49 × 105 Da. TFPI exhibited strong reducing power and possessed not only remarkable scavenging activities against ABTS•+ and DPPH radicals, but also high antitumor activities in C4-2, DU145, and PC3 cells. The results suggest that Trichosanthes Fructus and TFPI could be a novel potent natural medicine with antioxidant and antitumor activities.