Research Article

Txndc9 Is Required for Meiotic Maturation of Mouse Oocytes

Figure 3

Txndc9 knockdown resulted in aberrant spindle morphology in immature and mature mouse oocytes. Representative confocal images showed the spindle morphology and chromosome alignment of immature oocytes (a) and mature MII oocytes (c) by immunostaining with α-tubulin to visualize spindle (green) and nucleus (DAPI, blue). Quantification of negative control and Txndc9-siRNA oocytes with spindle/chromosome defects in immature (b) and mature (d) oocytes, and values are shown as means ± SD; , . Western blots showed the protein level of α-tubulin (e) and β-tubulin (f) in si-Txndc9 and negative control (NC) group.
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