Self-Conjugation of the Enteropathogenic Escherichia coli Adherence Factor Plasmid of Four Typical EPEC Isolates

Table 2

Primers used in this study.

Primer name	Region	Sequence 5′ to 3′	Reference
pEAF
bfpA_114F	bfpA	GTCTGCGTGTGATTCCAATA	[35]
bfpA_521R		TCAGCAGGAGTAATAGC	[35]
FIIA-F	repFIIA	CCTTCACACGACGTTCCACT	This study
FIIA-R		CGCCAGGTAAAGAACCCGAA	This study
traI-F	traI	GAGCTGGGTAAAGAGCAGGTCATGG	This study
traI-R		CAGGTTTGTTCTCTGCCATTTT	This study
traC-F	traC	GTCGGGRAGATGATTAACCATAA	This study
traC-R		ACCRACYTTRYGATTTTTGAAGTC	This study
ISSfl1-F	ISSfl1	TCGCCTCATGGGTAATGGGG	This study
ISSfl1-R		AATGGCGAGTGTCGAAGAACA	This study
Mutagenesis
ISSf11-H1	ISSfl1	CATGTCCTTCGTGCCAGCCTTCTGTGTGACGGGCGTTCCAT	This study
		TGTAGGCTGGAGCTGCTTCG
ISSf11-H2		ACCGAGTAACCACATTACGATAGTGCTCAACGTTGCCAGCAG	This study
		CATATGAATATCCTCCTTAG
K1	kan	CAGTCATAGCCGAATAGCCT	[38]
K2		CGGCCACAGTCGATGAATCC	[38]