Asymmetric Dimethylarginine Induced Apoptosis and Dysfunction of Endothelial Progenitor Cells: Role of Endoplasmic Reticulum Stress Pathway

Figure 4

ADMA-induced dysfunction of EPCs. (a) CCK-8 assay was performed to examine the proliferation of EPCs. Compared with the control group, ADMA (5~50 μmol/L) treatment significantly inhibited EPCs proliferation. (b and c) Transwell chemotaxis assay was performed to examine the migration of EPCs. ADMA (10 and 50 μmol/L) treatment inhibited EPCs migration, but 5 μmol/L ADMA did not. (d) ADMA (10 and 50 μmol/L) decreased the number of adherent EPCs. (b and e) ADMA (10 and 50 μmol/L) decreased EPCs tube formation (n = 3 per group). Mean ± SEM. versus control.

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