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BioMed Research International
Volume 2017, Article ID 6712742, 9 pages
https://doi.org/10.1155/2017/6712742
Research Article

Optimization, Purification, and Starch Stain Wash Application of Two New α-Amylases Extracted from Leaves and Stems of Pergularia tomentosa

1Laboratory of Plant Biodiversity and Dynamics of Ecosystems in Arid Environment, Faculty of Sciences, University of Sfax, Sfax, Tunisia
2Laboratory of Plant Biotechnology Applied to Crop Improvement, Faculty of Sciences, University of Sfax, Sfax, Tunisia
3Laboratory of Molecular Biotechnology of Eukaryotes, Center of Biotechnology, Sfax, Tunisia

Correspondence should be addressed to Imen Lahmar; rf.oohay@remhal.nemi

Received 24 August 2017; Accepted 27 November 2017; Published 17 December 2017

Academic Editor: Pierluigi Di Ciccio

Copyright © 2017 Imen Lahmar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

A continuous research is attempted to fulfil the highest industrial demands of natural amylases presenting special properties. New α-amylases extracted from stems and leaves of Pergularia tomentosa, which is widespread and growing spontaneously in Tunisia, were studied by the means of their activities optimization and purification. Some similarities were recorded for the two identified enzymes: (i) the highest amylase activity showed a promoted thermal stability at 50°C; (ii) the starch substrate at 1% enhanced the enzyme activity; (iii) the two α-amylases seem to be calcium-independent; (iv) Zn2+, Cu2+, and Ag2+ were considered as important inhibitors of the enzyme activity. Following the increased gradient of elution on Mono Q-Sepharose column, an increase in the specific activity of 11.82-fold and 10.92-fold was recorded, respectively, for leaves and stems with the presence of different peaks on the purification profiles. Pergularia amylases activities were stable and compatible with the tested commercial detergents. The combination of plant amylase and detergent allowed us to enhance the wash performance with an increase of 35.24 and 42.56%, respectively, for stems and leaves amylases. Characterized amylases were reported to have a promoted potential for their implication notably in detergent industry as well as biotechnological sector.