First Characterization of the Neospora caninum Dense Granule Protein GRA9

Figure 1

(a) In silico analysis reveals that the genomic sequence of NcGRA9 consists of one intron and two exons. The scheme depicts the genomic organization and the cDNA structure of NcGRA9. (b) The existence of the intron was confirmed by PCR on genomic DNA and cDNA with two different primer pairs (pp1 and pp2). The PCR products had the expected size by amplification with pp1 of 1697 bp (genomic DNA) or 957 bp (cDNA) and with pp2 of 1019 bp (genomic DNA) or 278 bp (cDNA).

(a)

(b)