First Characterization of the Neospora caninum Dense Granule Protein GRA9

Figure 2

NcGRA9 is a single-copy gene in the haploid tachyzoite genome of N. caninum. (a) depicts a schematic representation of the genomic region flanking Ncgra9 on chromosome XII. Restriction endonuclease recognition sites which are not placed in the exons are indicated. The probe used for Southern Blotting recognizes a sequence in the second exon. (b) shows Southern Blot analyses after enzymatic restriction of genomic DNA with different restriction endonucleases. In each case the probe detects a single band which implies that NcGRA9 is a single-copy gene in the haploid tachyzoite genome of N. caninum.

(a)

(b)