First Characterization of the Neospora caninum Dense Granule Protein GRA9

Table 1

Oligonucleotides used in this study.

1-1697for	5′-CACCACATGATGAGGTCATTCAAG-3′
1-1697rev	5′-CACCACTTATATTTCTCCGTTATGGTT-3′
271-1290for	5′-CACCACGTTCAGGAGATGATGCGG-3′
271-1290rev	5′-CACCACGACTCTCCCCGTGCT-3′
probeNcGRA9for	5′-CGTGCGCGTGGTGTTGCACTT-3′
probeNcGRA9rev	5′-TATTTCTCCGTTATGGTTCGAGAAACG-3′
NcGRA9NdeIfor	5′-CACCACCATATGATGATGAGGTCATTCAAG-3′
NcGRA9HindIIIre	5′-ATAATAAAGCTTTATTTCTCCGTTATGGTTCGAGAA-3′
pDHFRSpeINotIfo	5′-CACCACACTAGTAAGCTTCGCGAGGCTGTAAATCCCGTG-3′
pDHFRSpeINotIre	5′-ATAATAGCGGCCGCCCATGGCCTAGGCTTCCCGGAT-3′
GRA9HAAvrNcofor	5′-CACCACCCTAGGATGATGAGGTCATTCAAGTCC-3′
GRA9HAAvrNcorev	5′-ATAATACCATGGTTAAGCGTAATCTGGAACATCGTATGGGTATATTTCTCCGTT-3′
3′GRA9NcoINotIf	5′-CACCACCCATGGGCGCCACGTGTA-3′
3′GRA9NcoINotIr	5′-ATAATAGCGGCCGCTTCGGAACACACTACG-3′
NcGRA9d280revHA	5′-ATAATACCATGGTTAAGCGTAATCTGGAACATCGTATGGGTACTCTGTAGGAGT-3′
NcGRA9d201revHA	5′-ATAATACCATGGTTAAGCGTAATCTGGAACATCGTATGGGTAGCACTCAGGGTC-3′
Nc5forqPCR	5′-CGAGAGTTCAGTGTTCTGTGTTGA-3′
Nc5revqPCR	5′-TCGTCCGCTTGCTCCCTAT-3′
Nc5 Probe	FAM-CAACACCGGCGGCACTGATGA-BHQ1