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BioMed Research International
Volume 2017, Article ID 6747482, 8 pages
https://doi.org/10.1155/2017/6747482
Research Article

Recombinant Lactococcus lactis Expressing Haemagglutinin from a Polish Avian H5N1 Isolate and Its Immunological Effect in Preliminary Animal Trials

Institute of Biochemistry and Biophysics, Polish Academy of Sciences, Warsaw, Poland

Correspondence should be addressed to Agnieszka K. Szczepankowska; lp.waw.bbi@pezczsaga

Received 19 December 2016; Revised 20 January 2017; Accepted 24 January 2017; Published 22 February 2017

Academic Editor: Bernd H. Rehm

Copyright © 2017 Agnieszka K. Szczepankowska et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplementary Figure 1. Specific serum antibody response in mice ingested with L. lactis NZ-H5 cells. Days on which mice (n = 10) were immunized with 109 CFU of bacteria are indicated by arrows. H5-specific IgG (A) and IgA (B) titers were measured in serum samples collected on days 9, 17, 25 and 41 at 1:25 dilution. Graphs show results obtained for each immunized mice at given time point of blood sampling (diamonds). Data was normalized by adapting the cut-off (point 0 on the axis) as mean OD readings higher by 2 SD than for control mice. Supplementary Figure 2. Specific serum antibody response in mice ingested with L. lactis L-H5 cell lysates. Days on which mice (n = 10) were immunized with 109 CFU of bacteria are indicated by arrows. H5-specific IgG (A) and IgA (B) titers were measured in serum samples collected on days 9, 17, 25 and 41 at 1:25 dilution. Graphs show results obtained for each immunized mice at given time point of blood sampling (diamonds). Data was normalized by adapting the cut-off (point 0 on the axis) as mean OD readings higher by 2 SD than for control mice.

  1. Supplementary Material
  2. Supplementary Material