Peripheral Inhibitor of AChE, Neostigmine, Prevents the Inflammatory Dependent Suppression of GnRH/LH Secretion during the Follicular Phase of the Estrous Cycle

Figure 2

Effect of lipopolysaccharide (LPS; 400 ng/kg; iv.) and acetylcholinesterase inhibitors: Donepezil (2.5 mg/animal; iv.) and Neostigmine (0.5 mg/animal; iv.) injections on blood concentration of stress markers: cortisol (a) and prolactin (b) concentration in the blood plasma. The data are presented as the mean value ± SEM. All experiments consisted of a baseline period when no treatment was given (2 to 0.5 h before) and a period after treatment (1 to 3 h after). : asterisk indicates statistically significant differences between the period when no treatment was given and a period after treatment according to Student’s -test for dependent samples (“repeated measures”). The results of blood hormones concentration obtained only after treatment period were analysed using a two-way ANOVA. Different capital letters indicate significant differences according to a two-way ANOVA followed by Fisher’s post hoc test. Statistical significance was defined as .

(a)

(b)