Extracellular Signal-Regulated Kinase 5 is Required for Low-Concentration H2O2-Induced Angiogenesis of Human Umbilical Vein Endothelial Cells
Construction of high or low p-ERK5 HUVEC populations. (a) Images of lentivirus-transfected HUVECs. (b) ERK5, p-ERK5, MEF2C, p-MEF2C, ERK1/2, and p-ERK1/2 protein levels were determined by western blotting. Different cell populations were lysed, and the lysates were resolved by 10% or 6% SDS-PAGE. (c, d, e, f, g, h) Densitometric quantification of ERK5, p-ERK5, MEF2C, p-MEF2C, ERK1/2, and p-ERK1/2 expression (mean ± SD of three separate experiments, , and one-way ANOVA followed by SNK post hoc test).