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BioMed Research International
Volume 2017, Article ID 7039245, 14 pages
https://doi.org/10.1155/2017/7039245
Research Article

Mining of Microbial Genomes for the Novel Sources of Nitrilases

1Sub-Distributed Information Centre, Himachal Pradesh University, Summer Hill, Shimla 171005, India
2Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla 171005, India

Correspondence should be addressed to Tek Chand Bhalla; moc.liamffider@ctallahb

Received 25 October 2016; Revised 14 February 2017; Accepted 7 March 2017; Published 12 April 2017

Academic Editor: Jiangke Yang

Copyright © 2017 Nikhil Sharma et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Next-generation DNA sequencing (NGS) has made it feasible to sequence large number of microbial genomes and advancements in computational biology have opened enormous opportunities to mine genome sequence data for novel genes and enzymes or their sources. In the present communication in silico mining of microbial genomes has been carried out to find novel sources of nitrilases. The sequences selected were analyzed for homology and considered for designing motifs. The manually designed motifs based on amino acid sequences of nitrilases were used to screen 2000 microbial genomes (translated to proteomes). This resulted in identification of one hundred thirty-eight putative/hypothetical sequences which could potentially code for nitrilase activity. In vitro validation of nine predicted sources of nitrilases was done for nitrile/cyanide hydrolyzing activity. Out of nine predicted nitrilases, Gluconacetobacter diazotrophicus, Sphingopyxis alaskensis, Saccharomonospora viridis, and Shimwellia blattae were specific for aliphatic nitriles, whereas nitrilases from Geodermatophilus obscurus, Nocardiopsis dassonvillei, Runella slithyformis, and Streptomyces albus possessed activity for aromatic nitriles. Flavobacterium indicum was specific towards potassium cyanide (KCN) which revealed the presence of nitrilase homolog, that is, cyanide dihydratase with no activity for either aliphatic, aromatic, or aryl nitriles. The present study reports the novel sources of nitrilases and cyanide dihydratase which were not reported hitherto by in silico or in vitro studies.