BioMed Research International / 2017 / Article / Tab 3

Research Article

Mining of Microbial Genomes for the Novel Sources of Nitrilases

Table 3

Manually designed motifs (MDMs) for aliphatic and aromatic nitrilases showing the presence of essential catalytic triad (E, K, and C).

NitrilasesManually designed motif

Aliphatic[FL]-[ILV]-[AV]-F-P-E-[VT]-[FW]-[IL]-P-[GY]-Y-P-[WY]
R-R-K-[LI]-[KRI]-[PA]-T-[HY]-[VAH]-E-R
C-W-E-H-[FLX]-[NQ]-[PT]-L
[VA]-A-X-[AV]-Q-[AI]-X-P-[VA]-X-[LF]-[SD]

Aromatic[ALV]-[LV]-[FLM]-P-E-[AS]-[FLV]-[LV]-[AGP]-[AG]-Y-P
[AGN]-[KR]-H-R-K-L-[MK]-P-T-[AGN]-X-E-R
C-W-E-N-[HY]-M-P-[LM]-[AL]-R-X-X-[ML]-Y
A-X-E-G-R-C-[FW]-V-[LIV]