Mining of Microbial Genomes for the Novel Sources of Nitrilases

Table 4

Aliphatic and aromatic nitrilase motif patterns with bold letter depicting catalytic center (E, K, and C) in predicted nitrilases.

Nitrilases	Manually Designed motif	1	2	3	4	5	6	7	8	9
Aliphatic	[FL]-[ILV]-[AV]-F-P-E-[VT]-[FW]-[IL]-P-[GY]-Y-P-[WY]	A-F-P-E-V-F-V-P-A-Y-P-Y	F-P-E-L-W-L-P-G-Y-P-I-F	F-P-E-V-F-I-S-G-Y-P-Y-W-N-W				F-P-E-V-F-I-A-G-Y	F-P-E-T-F-V-P-Y-Y-P-Y
	R-R-K-[LI]-[KRI]-[PA]-T-[HY]-[VAH]-E-R	L-R-R-K-L-V-P-T-W	R-R-K-L-K-P-T-H-V-E-R	R-K-L-V-P-T-W-A-E-K-L-T				R-H-R-K-L-V-P-T-W-A-E-R	R-R-K-I-T-P-T-Y-H-E-R
	C-W-E-H-[FLX]-[NQ]-[PT]-L	C-G-E-N-T-N-T-L-A	C-A-E-N-M-Q-P-L	C-G-E-N-T-N-T-L-A				C-G-E-N-T-N-T-L-A-R-F-S	C-W-E-H-Y-N-P-L-
	[VA]-A-X-[AV]-Q-[AI]- X- P-[VA]-X-[LF]-[SD]	V-A-A-V-Q-A-A-P-V-F-L-D-P	V-A-S-V-Q-A-E	V-Q-T-A-P-V-F-L-N-V-E				A-A-V-Q-A-A-P-V-F-L	A-A-V-Q-I-S-P-V-L-
Aromatic	[ALV]-[LV]-[FLM]-P-E-[AS]-[FLV]-[LV]-[AGP]-[AG]-Y-P				F-Q-E-V-F-N-A	P-E-S-F-I-P-C-Y-P-R-G	F-P-E-A-F-L-G-T-Y-P			S-E-T-F-S-T-G
	[AGN]-[KR]-H-R-K-L-[MK]-P-T-[AGN]-X-E-R				R-K-H-H-I-P-Q-V	H-R-K-L-K-P-T-G-L-E-R	H-R-K-V-M-P-T-G-A-E-R			R-K-L-H-P-F-T
	C-W-E-N-[HY]-M-P-[LM]-[AL]-R-X-X-[ML]-Y				C-Y-D-R-H	C-W-E-N-Y-M-P-L-A-R-M	C-W-E-N-Y-M-P-L-L-R-A			C-Y-D-L-R-F-A
	A-X-E-G-R-C-[FW]-V-[LIV]				A-H-L-W-K-L-E	A-L-E-G-R-C-F-V-L-A	A-L-E-G-R-C-W-V			A-I-E-N-Q-A-Y-V