BioMed Research International / 2017 / Article / Tab 4

Research Article

Mining of Microbial Genomes for the Novel Sources of Nitrilases

Table 4

Aliphatic and aromatic nitrilase motif patterns with bold letter depicting catalytic center (E, K, and C) in predicted nitrilases.

NitrilasesManually Designed motif123456789

Aliphatic[FL]-[ILV]-[AV]-F-P-E-[VT]-[FW]-[IL]-P-[GY]-Y-P-[WY]A-F-P-E-V-F-V-P-A-Y-P-YF-P-E-L-W-L-P-G-Y-P-I-FF-P-E-V-F-I-S-G-Y-P-Y-W-N-WF-P-E-V-F-I-A-G-YF-P-E-T-F-V-P-Y-Y-P-Y
R-R-K-[LI]-[KRI]-[PA]-T-[HY]-[VAH]-E-RL-R-R-K-L-V-P-T-WR-R-K-L-K-P-T-H-V-E-RR-K-L-V-P-T-W-A-E-K-L-TR-H-R-K-L-V-P-T-W-A-E-RR-R-K-I-T-P-T-Y-H-E-R
C-W-E-H-[FLX]-[NQ]-[PT]-LC-G-E-N-T-N-T-L-AC-A-E-N-M-Q-P-LC-G-E-N-T-N-T-L-AC-G-E-N-T-N-T-L-A-R-F-SC-W-E-H-Y-N-P-L-
[VA]-A-X-[AV]-Q-[AI]- X- P-[VA]-X-[LF]-[SD]V-A-A-V-Q-A-A-P-V-F-L-D-PV-A-S-V-Q-A-EV-Q-T-A-P-V-F-L-N-V-EA-A-V-Q-A-A-P-V-F-LA-A-V-Q-I-S-P-V-L-

Aromatic[ALV]-[LV]-[FLM]-P-E-[AS]-[FLV]-[LV]-[AGP]-[AG]-Y-PF-Q-E-V-F-N-AP-E-S-F-I-P-C-Y-P-R-GF-P-E-A-F-L-G-T-Y-PS-E-T-F-S-T-G
[AGN]-[KR]-H-R-K-L-[MK]-P-T-[AGN]-X-E-RR-K-H-H-I-P-Q-VH-R-K-L-K-P-T-G-L-E-RH-R-K-V-M-P-T-G-A-E-RR-K-L-H-P-F-T
C-W-E-N-[HY]-M-P-[LM]-[AL]-R-X-X-[ML]-YC-Y-D-R-HC-W-E-N-Y-M-P-L-A-R-MC-W-E-N-Y-M-P-L-L-R-AC-Y-D-L-R-F-A
A-X-E-G-R-C-[FW]-V-[LIV]A-H-L-W-K-L-EA-L-E-G-R-C-F-V-L-AA-L-E-G-R-C-W-VA-I-E-N-Q-A-Y-V