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BioMed Research International
Volume 2017 (2017), Article ID 7157923, 8 pages
https://doi.org/10.1155/2017/7157923
Research Article

Integron-Mediated Antibiotic Resistance in Acinetobacter baumannii Isolated from Intensive Care Unit Patients, Babol, North of Iran

1Department of Microbiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
2Department of Medical Biotechnology, Faculty of Advanced Technologies in Medicine (SATiM), Tehran University of Medical Sciences, Tehran, Iran
3Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran
4Department of Biostatistics and Epidemiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
5Department of Microbiology, Faculty of Medicine, Iran University of Medical Sciences, Tehran, Iran

Correspondence should be addressed to Ramazan Rajabnia; moc.oohay@96nazamar

Received 6 December 2016; Revised 12 February 2017; Accepted 27 February 2017; Published 19 July 2017

Academic Editor: Paul M. Tulkens

Copyright © 2017 Mitra Deylam Salehi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. We investigated the integron types and their relation with antibiotic resistance among A. baumannii isolates collected from intensive care unit patients, Babol, north of Iran. Methods. In this cross-sectional study, a total of 73 bronchoalveolar lavage samples were obtained from patients in ICU. Susceptibility testing was performed by disk diffusion method. Types of integrons were identified by an integrase gene PCR. Results. In total, 47.9% A. baumannii isolates were recovered from the BAL samples. All isolates were resistant to ceftazidime. 91.4% and 58.3% of isolates were MDR and XDR, respectively. The rate of colistin resistance with the -test was 5.7%. Molecular analysis of class I, II, and III integrons showed that 25.7%, 88.6%, and 28.6% of the isolates carried the intI, intII, and intIII genes, respectively. Discussion. Our results show that different classes of integrons are commonly spread among A. baumannii strains and these genomic segments can play an important role in the acquisition of MDR and XDR phenotypes. So monitoring drug resistance in A. baumannii isolates with the use of int gene PCR is very important to plan specific infection control measures to prevent the spread of MDR-AB and XDR-AB in Iran’s hospitals.