Renal Expression and Urinary Excretion of Na-K-2Cl Cotransporter in Obstructive Nephropathy

Figure 2

Immunohistochemistry for NKCC2 in cortex and medulla kidney of sham (a) and BUO (b–d) rats. Serial sections from each rat kidney were stained with noncommercial polyclonal anti-NKCC2 antibodies as described in Section 2. Immunoperoxidase microscopy demonstrated association of NKCC2 labeling with apical membrane of thick ascending limb of Henle’s loop of sham rats (a). No difference in NKCC2 labeling was found in cortex of BUO-1 (b) and BUO-2 (c); increased labeling was seen in apical membrane in BUO-7 (d) kidneys. NKCC2 labeling in renal medullae was reduced in BUO-1 (b) and increased apical localization of NKCC2 in BUO-2 and BUO-7 (c-d) was observed. These figures are representatives of typical samples from four rats. Magnification 400.

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