Renal Expression and Urinary Excretion of Na-K-2Cl Cotransporter in Obstructive Nephropathy

Figure 3

mRNA levels of NKCC2 cotransporter in cortex (a) and medulla (b) of sham and BUO rats. Total RNA was isolated and NKCC2 and 18S mRNA were assessed by RT-PCR analysis. Relative mRNA levels were quantitated, and NKCC2 levels were normalized to 18S mRNA. Ratios are represented in graphical form, and the data are representative of four experiments. versus sham, versus BUO-1, versus BUO-2, and versus BUO-7.

(a)

(b)