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Figure 1: Time course and location of NR2B tyrosine phosphorylation (NR2B-pTyr) in TNC in CM rats. (a, b) Representative western blots of NR2B-pTyr and NR2B. NR2B-pTyr expression increased significantly after 24 and 48 hours and the level of NR2B was not changed significantly in CM rats. (c) Immunofluorescence double staining of NR2B-pTyr (red) and neuronal nuclei (NeuN, green) showed that the expression of NR2B-pTyr was increased and localized in TNC in CM. (d) Paw threshold was decreased in CM rats significantly. per group for western blots and threshold; per group for immunohistochemistry. versus sham. Bars: 100 μm.