A Novel Capillary Electrophoresis-Based High-Throughput Multiplex Polymerase Chain Reaction System for the Simultaneous Detection of Nine Pathogens in Swine

Table 1

Primers information of QIAxcel assay.

Name	Sequence (5′-3′)	Gene	Size range (bp)
PRV	F: AGGTGACACTATAGAATAAGGGGTTGGACAGGAAGGACA	gB	161
	R: GTACGACTCACTATAGGGACATCGCCAACTTCTTCCAGG
PRRSV	F: AGGTGACACTATAGAATACATCGCCCAACAAAACCAGTCC	N	183
	R: GTACGACTCACTATAGGGAACGACAGACACAATTGCCGCTC
JEV	F: AGGTGACACTATAGAATATGGCTCTATTGGAGGGGTCT	E	197
	R: GTACGACTCACTATAGGGAAATTGATCGGTCTCGTGCGT
FMDV	F: AGGTGACACTATAGAATACCGACAAAAGCGACAAAGGTT	3D	211
	R: GTACGACTCACTATAGGGAATCAACTTCTCCTGTATGGTCCC
CSFV	F: AGGTGACACTATAGAATATGGCAAATGAGACAGGTTACAGA	E2	229
	R: GTACGACTCACTATAGGGATCCTTACAGGTCCCGCACTA
PCV-2	F: AGGTGACACTATAGAATAGGAAGAAGCGGACCCCAACC	ORF1	255
	R: GTACGACTCACTATAGGGAAGCGGGCACCCAAATACCA
TGEV	F: AGGTGACACTATAGAATAGGAACTTATGTCCGAGAGACTTTG	N	295
	R: GTACGACTCACTATAGGGAGGATTCATTATTAGCACCACGACT
PEDV	F: AGGTGACACTATAGAATAACTAATAAGGGGAATAAGGACCA	N	317
	R: GTACGACTCACTATAGGGAAACAATCTCAACTACACTGGGGA
PPV	F: AGGTGACACTATAGAATAATACTTGGGGGAGGGCTTGG	VP1	363
	R: GTACGACTCACTATAGGGATTGGTGGTGAGGTTGCTGAT
SP	F: AGGTGACACTATAGAATA
	R: GTACGACTCACTATAGGGA

Sequence: the underline means label sequences.