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BioMed Research International
Volume 2017 (2017), Article ID 7283806, 15 pages
https://doi.org/10.1155/2017/7283806
Research Article

Chemical Compositions, Somatic Embryogenesis, and Somaclonal Variation in Cumin

1Department of Agronomy and Plant Breeding, College of Aburaihan, University of Tehran, Tehran, Iran
2Department of Plant Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, G.C., Tehran, Iran
3Facoltà di Agraria, Università degli Studi di Napoli Federico II, Portici, Italy

Correspondence should be addressed to Seyed Mohammad Mahdi Mortazavian

Received 20 May 2017; Revised 16 August 2017; Accepted 9 October 2017; Published 6 November 2017

Academic Editor: Hely M. Häggman

Copyright © 2017 Moslem Bahmankar et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This is the first report evaluating the relationship between the chemical compositions of cumin seeds (based on the analysis of the content of catalase, ascorbate peroxidase, proline, protein, terpenic compounds, alcohol/phenols, aldehydes, and epoxides) and the induction efficiency of somatic embryogenesis in two Iranian superior cumin landraces (Golestan and North Khorasan). Cotyledons isolated from Golestan landrace seeds cultivated on MS medium supplemented with 0.1 mg/L kinetin proved to be the best primary explant for the induction of somatic embryogenesis as well as the regeneration of the whole plantlet. Results indicated that different developmental stages of somatic embryos were simultaneously observed on a callus with embryogenic potential. The high content of catalase, ascorbate peroxidase, proline, and terpenic hydrocarbons and low content of alcoholic and phenolic compositions had a stimulatory effect on somatic embryogenesis. Band patterns of RAPD markers in regenerated plants were different from those of the mother plants. This may be related to somaclonal variations or pollination system of cumin. Generally, measurement of chemical compositions can be used as a marker for evaluating the occurrence of somatic embryogenesis in cumin. Also, somaclonal variations of regenerated plants can be applied by the plant breeders in breeding programs.