Research Article

Differentiation of Human Amniotic Mesenchymal Stem Cells into Human Anterior Cruciate Ligament Fibroblast Cells by In Vitro Coculture

Figure 7

Collagen expression on day 21 and its quantification in each group based on picrosirius red staining. The results of picrosirius red staining showed total collagen secretion in each group; collagen was mainly produced intracellularly, and some secretion into the cytoplasm was observed. Group E showed a significantly increased level on day 21. Group F showed a lesser degree of collagen secretion than Group E. Total collagen expression in Group C was significantly increased on days 7, 14, and 21 and was higher than that in Groups A and B. Group A showed higher collagen secretion on days 7, 14, and 21 compared to Group B, which showed limited collagen production and fixed expression at the same time points (original magnification ×100, scale bar = 100 μm) ().