Research Article

GosB Inhibits Triacylglycerol Synthesis and Promotes Cell Survival in Mouse Mammary Epithelial Cells

Figure 4

GosB affects milk fat synthesis-induced by PPARγ. The mouse mammary gland epithelial cells (MEC) were transfected with Ad-GosB or Ad-siGosB for 48 hours, followed by exposure to 10 μM rosiglitazone (ROSI) for 24 hours. Then the lipid-droplets accumulation was determined by Oil Red O staining assays (a) and quantified by measuring absorbance at 490 nm (b), and the intracellular triglyceride content was also measured (c). Values are means ± SEM for three individuals. .
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