Variety of RNAs in Peripheral Blood Cells, Plasma, and Plasma Fractions

Table 1

Human blood fractions obtained in the investigation.

Blood fraction	Description	Method of isolation	Characteristics of the method
(I) cells	Whole blood, enriched with blood cells	1,200, pellet	Preparation of platelet-poor plasma [19] and estimation of haemolysis [20]
(II) plasma	Platelet-poor blood plasma	1,200, supernatant
(III) 16,000 plasma pellets	Intermediate fraction containing membrane vesicles and cell debris	16,000, pellet	Isolation of microvesicles [21]
(IV) 160,000 plasma pellets	Fraction enriched with exosomes	160,000, pellet	High-recovery isolation of exosomes [22]
(V) 160,000 plasma supernatant	Vesicle-depleted plasma	160,000, supernatant

Centrifugation for 20 min at 4°C.
Centrifugation for 120 min at 4°C.