Aberrant Long Noncoding RNAs Expression Profiles Affect Cisplatin Resistance in Lung Adenocarcinoma

Figure 4

Some lncRNA expression in LAD and A549/DDP, A549 cell. (a) LncRNA-mRNA coexpression network, the results imply that UCA1, ENST00000443252, ENST00000510562, ENST00000565689, ENST00000558690, ENST00000397340, ENST00000440955, and ENST00000507916 are also closely related to many mRNA molecules. (b) Comparison between gene chip data and qPCR result. The validation results of the 12 lncRNAs indicated that the microarray data correlated well with the qPCR results. UCA1 expression level in LAD and A549/DDP, A549 cell. (c) The expression of UCA1 in cisplatin-resistant A549/DDP cells was significantly higher than that in cisplatin-sensitive A549 cells. (d) We used CCK-8 method to detect the sensitivity of A549/DDP cells to cisplatin. (e) The IC50 of cisplatin in UCA1 siRNA group was significantly lower than that in NC group, control group. (f) The IC50 of cisplatin in UCA1 overexpression group was significantly higher than that in NC group, control group.

(a)

(b)

(c)

(d)

(e)

(f)