Purification, Characterization, and Mode of Action of Pentocin JL-1, a Novel Bacteriocin Isolated from Lactobacillus pentosus, against Drug-Resistant Staphylococcus aureus

Table 3

Purification and activity of the bacteriocin produced by L. pentosus JL-1.

Samples	Total protein; (mg)	Total bacteriocin activity; (IU)	Specific activity (IU/mg)	Purification; (fold)	Yield; (%)
Supernatant	2690	100440	37	1.0	100.0
Macroporous resin D4020	460	49740	108	2.9	49.5
Cation exchange	47	20320	432	11.7	20.2
Gel chromatography	12	11090	924	25.0	11.0
C18 RP- HPLC	1.8	4710	2617	70.7	4.7