The PDZ-Binding Motif of HPV16-E6 Oncoprotein Modulates the Keratinization and Stemness Transcriptional Profile In Vivo

Figure 1

Global transcriptional analysis of K14E6 versus K14E6Δ146-151 mice. (a) Hierarchical clustering of genes differentially expressed in an RNA-microarray analysis performed on K14E6 versus K14E6Δ146-151 comparison. A -score adjusts heatmap. (b) Pathway enrichment plot of differentially expressed genes. The plot shows the significantly    value) upregulated (red bars) and downregulated (green bars) pathways of differentially expressed genes. (c) Networks of three of the most negatively enriched cellular processes (keratinocyte differentiation, stem cell maintenance, and keratinization) based on statistical significance ( value). (d) Microarray validation by RT-qPCR. Grey bars represent microarray expression value versus black bars which represent RT-qPCR validation; each RT-qPCR validation was performed as biological replicates.

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