Basal and Activated Calcium Sensitization Mediated by RhoA/Rho Kinase Pathway in Rats with Genetic and Salt Hypertension
Table 4
Kinetic analysis of BAY K8644 dose-response curves (shown in Figure ) recorded before and after fasudil administration in WKY and SHR rats and HanSD and TGR animals as well as in SS/Jr or SR/Jr Dahl rats fed either LS or HS diet (Experiment ).
(a)
Sigmoidal logistic model parameters
Dose-response curve to BAY K8644 before fasudil administration
Dose-response curve to BAY K8644 after fasudil administration
Two-way ANOVA value
Factor strain
Factor fasudil treatment
SHR
WKY
SHR + fasudil
WKY + fasudil
max
<0.001
<0.001
log ED50
ns
ns
slope
ns
ns
TGR
HanSD
TGR + fasudil
HanSD + fasudil
max
<0.001
<0.001
log ED50
ns
ns
slope
ns
ns
(b)
Sigmoidal logistic model parameters
Dose-response curve to BAY K8644 before fasudil administration
Dose-response curve to BAY K8644 after fasudil administration
Two-way ANOVA value
Factor salt intake
Factor fasudil treatment
SS/Jr-HS
SS/Jr-LS
SS/Jr-HS + fasudil
SS/Jr-LS + fasudil
max
ns
<0.001
log ED50
ns
ns
slope
ns
ns
SR/Jr-HS
SR/Jr-LS
SR/Jr-HS + fasudil
SR/Jr-LS + fasudil
max
ns
ns
log ED50
ns
ns
slope
ns
ns
Data are means ± SEM. max: maximal response (mmHg), log ED50: median effective dose (log dose µg/kg), and slope: slope of the dose-response curve (mmHg/ log dose µg/kg). Significantly different ()from the respective normotensive controls and #from the corresponding rats measured before fasudil administration.
