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BioMed Research International
Volume 2017, Article ID 8037963, 8 pages
https://doi.org/10.1155/2017/8037963
Research Article

Assessment of the Usefulness of Multiplex Real-Time PCR Tests in the Diagnostic and Therapeutic Process of Pneumonia in Hospitalized Children: A Single-Center Experience

1Department of Family Medicine, University of Medical Sciences, Poznan, Poland
2Department of Paediatric Anaesthesiology and Intensive Therapy, University of Medical Sciences, Poznan, Poland
3Department of Paediatric Radiology, University of Medical Sciences, Poznan, Poland
4Department of Medical Diagnostics, University of Medical Sciences, Dobra 38, Poznan, Poland
5Department of Paediatric Cardiology, University of Medical Sciences, Poznan, Poland
6Department of Paediatric Diabetology, University of Medical Sciences, Poznan, Poland
7Department of Paediatric Oncology, Hematology and Bone Marrow Transplantation, University of Medical Sciences, Poznan, Poland
8Department of Infectious Diseases, University of Medical Sciences, Poznan, Poland
9Department of Paediatric Pulmonology, University of Medical Sciences, Poznan, Poland
10Department of Health Promotion, University of Medical Sciences, Poznan, Poland
11Department of Molecular Pathology, Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland

Correspondence should be addressed to Ewelina Gowin; lp.teno.atzcop@ogewe

Received 1 September 2016; Revised 3 December 2016; Accepted 18 December 2016; Published 15 January 2017

Academic Editor: Adriana Ceci

Copyright © 2017 Ewelina Gowin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The aim of the study was assessment of the usefulness of multiplex real-time PCR tests in the diagnostic and therapeutic process in children hospitalized due to pneumonia and burdened with comorbidities. Methods. The study group included 97 children hospitalized due to pneumonia at the Karol Jonscher Teaching Hospital in Poznań, in whom multiplex real-time PCR tests (FTD respiratory pathogens 33; fast-track diagnostics) were used. Results. Positive test results of the test were achieved in 74 patients (76.3%). The average age in the group was 56 months. Viruses were detected in 61 samples (82% of all positive results); bacterial factors were found in 29 samples (39% of all positive results). The presence of comorbidities was established in 90 children (92.78%). On the basis of the obtained results, 5 groups of patients were established: viral etiology of infection, 34 patients; bacterial etiology, 7 patients; mixed etiology, 23 patients; pneumocystis, 9 patients; and no etiology diagnosed, 24 patients. Conclusions. Our analysis demonstrated that the participation of viruses in causing severe lung infections is significant in children with comorbidities. Multiplex real-time PCR tests proved to be more useful in establishing the etiology of pneumonia in hospitalized children than the traditional microbiological examinations.